Assessing the anti-tumour properties of Iraqi propolis in vitro and in vivo

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• 作者：Ghassan M. Sulaiman^a ; Ali H. Ad’ ; hiah^b ; Khulood W. Al-Sammarrae^c ; Renzo Bagnati^d ; Roberta Frapolli^e ; Ezia Bello^e ; Sarah Uboldi^e ; Michela Romano^e ; Nicolò ; Panini^e ; Eugenio Scanziani^f ; Marzia Pezzolato^g ; Eugenio Erba^e ; ^{eugenio.erba@marionegri.it} ; Maurizio D’ ; Incalci^e
• 关键词：Iraqi propolis ; Cytotoxicity ; BrdU/DNA cell cycle
• 刊名：Food and Chemical Toxicology
• 出版年：2012
• 期刊代码：20_02786915
• 类别：pha
• 出版时间：May, 2012
• 卷：50
• 期：5
• 页码：1632-1641
• 文件大小：2131 K

摘要

The study was designed to evaluate anti-tumour properties of Iraqi propolis collected from Mosul region (M) on HL-60 and HCT-116 cell lines and on HCT-116 m>in vivo.m> M induced an inhibitory effect against the proliferation of HL-60 and colony potential of HCT-116 cells. The apoptosis in HL-60 cells was associated with down-regulation of Bcl-2 and activation of Bax, while in HCT-116 cells, necrotic features were observed; size of cells was dramatically increased by swelling of cytoplasm and loss of membrane integrity, cell rupture and release of cellular contents. Analysis of BrdU/DNA cell cycle in both cell lines showed that M induced cell cycle perturbations in both BrdU positive and BrdU negative cells. The exposure of HL-60 to M caused 纬-H2AX in a dose dependent manner and was associated with induction of apoptosis. The experiments in HCT-116 tumor-bearing mice showed that oral administration of propolis at doses that caused no detectable toxicity was associated with a decrease in mitotic cells and an increase in endoreduplications, increased p53 and decreased Ki-67 expression of cells in tumor sections. This study provides the rationale to investigate the potential beneficial effect of propolis in the diet of patients receiving anti-cancer therapies.