Selective trace analysis of chloroacetamide herbicides in food samples using dummy molecularly imprinted solid phase extraction based on chemometrics and quantum chemistry

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• 作者：Lei Zhang^a ; ^b ; Fang Han^a ; ^b ; Yanyun Hu^a ; ^b ; Ping Zheng^a ; ^b ; ^{ahciq_hx@163.com} ; Xuan Sheng^a ; Hao Sun^a ; Wei Song^b ; Yaning Lv^b
• 关键词：Dummy template ; Dummy molecularly imprinted solid phase extraction ; Molecule modeling ; Clustering analysis ; Chloroacetamide herbicides
• 刊名：Analytica Chimica Acta
• 出版年：2012
• 期刊代码：2_00032670
• 类别：ch
• 出版时间：4 June, 2012
• 卷：729
• 期：Complete
• 页码：36-44
• 文件大小：676 K

摘要

A methodology based on chemometrics and quantum chemistry was proposed to design and synthesize dummy molecularly imprinted polymers (DMIPs) capable of extracting chloroacetamide herbicides from food samples. Molecular modeling approach in conjunction with clustering analysis was used to predict the most suitable dummy template. Metolachlor deschloro was selected as the template to synthesize DMIPs, which were used as the solid phase extraction (SPE) materials. The selective adsorption of DMIPs was evaluated by high performance liquid chromatography (HPLC). The retention property of six chloroacetamide herbicides on DMIPs was also predicted using clustering analysis. The optimum loading, washing and eluting conditions for dummy molecularly imprinted solid phase extraction (DMISPE) were established to obtain high selectivity and sensitivity. Water, dichloromethane and methanol were chosen as loading, washing and elution solvent, respectively. Under optimized DMISPE conditions, recoveries of analytes were in the range of 83.4-106.7%with satisfactory precision (RSD%lower than 13%). Compared with other commercial SPE columns, DMISPE exhibited selective binding properties for chloroacetamide herbicides and the matrix effect was significantly decreased.