Optimization of a solid phase extraction and hydrophilic interaction liquid chromatography-tandem mass spectrometry method for the determination of metformin in dietary supplements and herbal medicines

详细信息	查看全文 | 推荐本文 |

• 作者：Ximei Wu^a ; ^b ; ^{wxmdwb927@126.com} ; Binghui Zhu^a ; Lin Lu^a ; Weixiong Huang^a ; Daiwen Pang^b ; ^{dwpang@whu.edu.cn}
• 关键词：Metformin ; HILIC/MS/MS ; Dietary supplements ; Herbal medicines ; Solid phase extraction ; Primary&ndash ; secondary amine adsorbent
• 刊名：Food Chemistry
• 出版年：2012
• 期刊代码：48_03088146
• 类别：abs
• 出版时间：15 July, 2012
• 卷：133
• 期：2
• 页码：482-488
• 文件大小：684 K

摘要

Metformin is one of the most common adulterants found in anti-diabetic dietary supplements and herbal medicines. An analytical method based on hydrophilic interaction chromatography (HILIC)/tandem mass spectrometry was developed and validated for the determination of metformin in dietary supplements and herbal medicines. Sample preparation involved solid phase extraction of the analyte using Plexa PCX cartridges, and cleanup by a primary-secondary amine (PSA) adsorbent for minimization of the matrix effect. Chromatographic separation was performed on a HILIC column using a mixture of 0.025 mol/L ammonium formate (pH 3) and acetonitrile (18:82, v/v) as the mobile phase at flow rate of 0.25 mL/min. The method was validated in four matrices (capsules, honeyed pills, tablets and oral solutions). The method had a good linear range (5-500 ng/mL), and the overall precision and accuracy ranged from 2.2%to 9.9%and 4.6%to 11.3%, respectively.