A sensitive and selective HPLC-FLD method with fluorescent labeling for simultaneous detection of bile acid and free fatty acid in human serum

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• 作者：Guo-Liang Li^a ; ^{guolianglibest@yahoo.com.cn} ; Guang Chen^a ; ^b ; Yue-Qiu Liu^c ; Nian-Hua Jing^b ; Jin-Mao You^a ; ^b ; ^{jmyou6304@163.com}
• 关键词：Bile acids ; Free fatty acids ; Human serum ; Fluorescent labeling ; HPLC-FLD
• 刊名：Journal of Chromatography B ; Analytical Technologies in the Biomedical and Life Sciences
• 出版年：2012
• 期刊代码：124_15700232
• 类别：ch
• 出版时间：1 May, 2012
• 卷：895-896
• 期：Complete
• 页码：191-195
• 文件大小：512 K

摘要

A sensitive and selective method using 2-(7H-dibenzo[a,g]carbazol-7-yl)ethyl 4-methylbenzenesulfonate (DBCETS) as a new fluorescent labeling reagent has been proposed for simultaneously detecting BA and FFA by HPLC with fluorescence detector. The developed method offered the low detection limits of 0.42-0.70 and 0.28-0.57 ng/mL for BA and FFA, respectively. Compared with the reported methods, the proposed method here is capable of offering higher detection sensitivity and selectivity, with less cost and lower volume of sample preparation. This method was validated to ensure high accuracy and precision, and the reliability of its results. When applied to the serum samples of healthy volunteers and patients with hepatic carcinoma, it showed excellent applicability.