Glucose levels regulate the nucleo-mitochondrial distribution of Mig2

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• 作者：Alejandra Ferná ; ndez-Cid¹ ; Alberto Riera¹ ; Pilar Herrero ; Fernando Moreno ; ^{fmoreno@uniovi.es}
• 关键词：HA ; haemagglutinin ; Myc ; c-Myc peptide epitope ; G6PD ; glucose-6-phosphate dehydrogenase ; GFP ; green fluorescent protein ; RFP ; red fluorescent protein ; DAPI ; 4&prime ; 6-Diamino-2-phenylindole ; SD ; synthetic dextrose ; SGly ; synthetic glycerol ; YEPD ; yeast e
• 刊名：Mitochondrion
• 出版年：2012
• 期刊代码：39_15677249
• 类别：neu
• 出版时间：May, 2012
• 卷：12
• 期：3
• 页码：370-380
• 文件大小：1492 K

摘要

Mig2 has been described as a transcriptional factor that in the absence of Mig1 protein is required for glucose repression of the SUC2 gene. Thus, until now, the main role assigned to Mig2 has been the functional redundancy to Mig1. In this study, we report that Mig2 has a double subcellular localization. As expected, in high-glucose conditions it is accumulated in the nucleus but in low-glucose conditions Mig2 has an unexpected mitochondrial localization and role in mitochondrial morphology. We describe that Mig2 physically interacts with the mitochondrial protein Ups1 in a glucose-dependent manner. We also show that 螖mig2 mutant cells exhibit a fragmented network of mitochondrial tubules, a phenotype similarly observed in cells lacking Fzo1 and Ups1. Furthermore, Mig2 acts antagonistically with respect to the fission-promoting components, because mitochondrial aggregation induced by DNM1 deletion was rescued in the 螖dnm1螖mig2 double mutant. Thus, our studies have revealed an additional role for Mig2 as a novel factor required for the maintenance of fusion-competent mitochondria in Saccharomyces cerevisiae and strongly suggest that Mig2 could be involved in the cross talk between the nucleus and the mitochondria through Ups1 to regulate mitochondrial morphology in a glucose dependent manner.