Effects of autogenous growth factors on heterotopic bone formation of osteogenic cells in small animal model

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• 作者：Premjit Arpornmaeklong^a ; ^{premjit.a@psu.ac.th} ; Prisana Pripatnanont^a ; Winai Kittidumkerng^a ; Winyou Mitarnun^b
• 关键词：Platelet concentrate ; Bone marrow stromal cells ; Autologous cell transfer ; Bone formation
• 刊名：Journal of Cranio-Maxillofacial Surgery
• 出版年：2012
• 期刊代码：166_10105182
• 类别：med
• 出版时间：June, 2012
• 卷：40
• 期：4
• 页码：332-340
• 文件大小：2216 K

摘要

Aims

This study used a new approach to investigate the effective concentrations of growth factors released from platelet concentrate (PC) on the bone formation capacity of osteogenically differentiated rat bone marrow stromal cells (rBMSCs).

Materials and methods

Rat BMSCs and whole blood were harvested from 40 adult male Spraque-Dawly rats. Rat BMSCs were expanded in an osteogenic medium and seeded on inert collagenous bovine bone matrix (ICBM). Growth factors released from degranulated PC (GFs) containing TGF-尾1 1 (25 ng/ml)-10 ng (250 ng/ml) and rhBMP-2 400 ng (10 渭g/ml) were suspended in 40 渭l platelet poor plasma (PPP) and applied on the ICBM-rBMSC constructs or ICBM only, respectively. The constructs were then transplanted in autologous hosts for 4 weeks. Concurrently, osteoblastic differentiation of rBMSCs on ICBM-rBMSC-PPP constructs was characterized in聽vitro.

Results

Rat BMSCs in osteogenic medium exhibited phenotypes of mature osteoblasts. The amount of newly formed bone among groups of ICBM-rBMSC-PPP with and without GFs was not significantly different (p > 0.05) and was significantly lower than a group of ICBM-PPP-BMP-2 (p < 0.05).

Conclusions

Autogenous GFs had no effect on the capacity of rBMSCs to form new bone. The ability to measure the bone formation capacity of transplanted autologous cells and growth factors in a small animal model was demonstrated.