The present review discusses several bioprocess engineering aspects related to P. pastoris cultivation, including the different promoters available, both constitutive and inductive, on- and off-line process parameter monitoring methods, fed-batch and continuous cultivation control strategies, proteolytic degradation of products and methods to minimize associated yield reductions, and the different models devised to describe cell growth and protein production. The economics of P. pastoris cultivation, through a case-study involving recombinant phytase production, are also discussed. The study of yeast fermentation is currently very dynamic, and P. pastoris is on its way towards becoming a standard expression system.
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