Potential allergenicity research of Cry1C protein from genetically modified rice

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• 作者：Sishuo Cao^a ; ¹ ; Xiaoyun He^a ; ^c ; ¹ ; Wentao Xu^a ; ^b ; ^{cauxwt@yahoo.cn} ; YunBo Luo^a ; Wenjun Ran^a ; Lixing Liang^a ; Yunqing Dai^a ; Kunlun Huang^a ; ^b ; ^{hkl009@163.com}
• 关键词：BN ; Brown Norway ; GM ; genetically modified ; OVA ; ovalbumin ; PAP ; potato acid phosphatase ; PBST ; phosphate-buffered saline containing 0.02%Tween 20 ; PNA ; peanut agglutinin ; TMB ; 3 ; 3&prime ; 5 ; 5&prime ; -tetramethylbenzidine
• 刊名：Regulatory Toxicology and Pharmacology
• 出版年：2012
• 期刊代码：57_02732300
• 类别：pha
• 出版时间：July, 2012
• 卷：63
• 期：2
• 页码：181-187
• 文件大小：987 K

摘要

With the development of genetically modified crops, there has been a growing interest in available approaches to assess the potential allergenicity of novel gene products. We were not sure whether Cry1C could induce allergy. We examined the protein with three other proteins to determine the potential allergenicity of Cry1C protein from genetically modified rice. Female Brown Norway (BN) rats received 0.1 mg peanut agglutinin (PNA), 1 mg potato acid phosphatase (PAP), 1 mg ovalbumin (OVA) or 5 mg purified Cry1C protein dissolved in 1 mL water by daily gavage for 42 days to test potential allergenicity. Ten days after the last gavage, rats were orally challenged with antigens, and physiologic and immunologic responses were studied. In contrast to sensitization with PNA, PAP and OVA Cry1C protein did not induce antigen-specific IgG2a in BN rats. Cytokine expression, serum IgE and histamine levels and the number of eosinophils and mast cells in the blood of Cry1C group rats were comparable to the control group rats, which were treated with water alone. As Cry1C did not show any allergenicity, we make the following conclusion that the protein could be safety used in rice or other plants.