Novel urchin-like In₂O₃-chitosan modified electrode for direct electrochemistry of glucose oxidase and biosensing

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• 作者：Zhanjun Yang ; ^{zjyang@yzu.edu.cn} ; Xiaochun Huang ; Rongcai Zhang ; Juan Li ; Qin Xu ; Xiaoya Hu ; ^{xyhu@yzu.edu.cn}
• 关键词：Urchin-like In2O3 ; Nanostructure ; Direct electrochemistry ; Glucose oxidase ; Glucose
• 刊名：Electrochimica Acta
• 出版年：2012
• 期刊代码：30_00134686
• 类别：ch
• 出版时间：30 May, 2012
• 卷：70
• 期：Complete
• 页码：325-330
• 文件大小：824 K

摘要

A novel urchin-like In₂O₃-chitosan modified glassy carbon electrode (GCE) is for the first time prepared. The direct electrochemistry of glucose oxidase (GOD) immobilized on the surface of modified GCE and biosensing are studied. The urchin-like In₂O₃ nanostructure-based matrix has large specific surface area and provides a favorable and biocompatible microenvironment for promoting the direct electron transfer between proteins and electrode surface. The properties of different modified electrode are characterized by scanning electron microscopy (SEM), electrochemical impedance spectra (EIS), UV-vis spectroscopy, Fourier transform infrared spectroscopy (FTIR) and cyclic voltammetry, respectively. The constructed glucose biosensor shows wide linear range (5.0 脳 10^鈭? to 1.3 脳 10^鈭? M), low detection limit (1.9 脳 10^鈭? M), a Michaelis-Menten constant of 0.37 mM. The proposed biosensor has good sensitivity, excellent selectivity, good reproducibility and stability. This urchin-like In₂O₃-chitosan matrix provides a new approach and efficient matrix for the direct electrochemistry of proteins and developing novel types of biosensors.