The effect of total-ABL, GUS and B2M control genes on BCR-ABL monitoring by real-time RT-PCR
- 作者：Jana Rulcová ; Vá ; clava Zmeková ; Zuzana Zemanová ; Hana Klamová ; ; Jana Moravcová
- 关键词：CML ; BCR-ABL monitoring ; Real-time RT-PCR ; Control genes ; Disease status ; Disease prognosis
- 刊名：Leukemia Research
- 出版年：2007
- 期刊代码：186_01452126
- 类别：med
- 出版时间：April 2007
- 卷：31
- 期：4
- 页码：483-491
- 文件大小：462 K
摘要
We compared the effect of control genes (CG): total Abelson (total-ABL), beta-2-microglobulin (B2M) and beta-glucuronidase (GUS), recommended in the Europe Against Cancer (EAC) program, on real-time BCR-ABL monitoring in patients with chronic myeloid leukemia (CML). We focused on the stability of CG expressions during therapy and the effect of the CGs on BCR-ABL ability to characterize the disease status and disease prognosis, issues that have not been addressed yet. The results showed B2M as a very convenient CG for BCR-ABL monitoring. On the contrary, the widely used total-ABL was not confirmed as appropriate for normalization of gene expression in CML.