Identification of a peptide sequence that improves transport of macromolecules across the intestinal mucosal barrier targeting goblet cells
- 作者：Sang Kee Kang ; Jung Hee Woo ; Min Kook Kim ; Sang Soo Woo ; Jin Hyuk Choi ; Hong Gu Lee ; Nam Kyung Lee ; Yun Jaie Choi
- 关键词：Phage display ; Intestinal mucosa ; Receptor-mediated endocytosis ; Transcytosis ; Oral drug delivery system ; Goblet cell
- 刊名：Journal of Biotechnology
- 出版年：2008
- 期刊代码：53_01681656
- 类别：imm
- 出版时间：1 June 2008
- 卷：135
- 期：2
- 页码：210-216
- 文件大小：2827 K
摘要
In this study, we demonstrated that the CSKSSDYQC-peptide ligand which was identified from a random phage-peptide library through an in vivo phage display technique with rats could prominently improve the transport efficiency of macromolecules, such as large filamentous phage particles (M13 bacteriophage), across the intestinal mucosal barrier. Synthetic CSKSSDYQC-peptide ligands significantly inhibited the binding of phage P1 encoding CSKSSDYQC-peptide ligands to the intestinal mucosal tissue and immunohistochemical analysis showed that the CSKSSDYQC-peptide ligands could be transported across the intestinal mucosal barrier via goblet cells as their specific gateway. Thus, we inferred that CSKSSDYQC-peptide ligand might have a specific receptor on the goblet cells and transported from intestinal lumen to systemic circulation by transcytosis mechanism. These results suggest that CSKSSDYQC-ligand could be a promising tool for development of an efficient oral delivery system for macromolecular therapeutics in the carrier-drug conjugate strategy.