Laccase immobilization on enzymatically functionalized polyamide 6,6 fibres
- 作者：Carla Silva ; Carla Joana Silva ; Andrea Zille ; Georg M. Guebitz ; Artur Cavaco-Paulo
- 关键词：Polyamide ; Nylon ; Immobilization ; Crosslinking ; Glutaraldehyde ; Laccase
- 刊名：Enzyme and Microbial Technology
- 出版年：2007
- 期刊代码：26_01410229
- 类别：ce
- 出版时间：1 November 2007
- 卷：41
- 期：6-7
- 页码：867-875
- 文件大小：543 K
摘要
Polyamide matrices, such as membranes, gels and non-wovens, have been applied as supports for enzyme immobilization, although in literature the enzyme immobilization on woven nylon matrices is rarely reported. In this work, a protocol for a Trametes hirsuta laccase immobilization using woven polyamide 6,6 (nylon) was developed. A 24 full factorial design was used to study the influence of pH, spacer (1,6-hexanediamine), enzyme and crosslinker concentration on the efficiency of immobilization. The factors enzyme dosage and spacer seem to have played a critical role in the immobilization of laccase onto nylon support. Under optimized working conditions (29 U mL−1 of laccase, 10%of glutaraldehyde, pH = 5.5, with the presence of the spacer), the half-life time attained was about 78 h (18%higher than that of free enzyme), the protein retention was 30%and the immobilization yield was 2%. The immobilized laccase has potential for application in the continuous decolourization of textile effluents, where it can be applied into a membrane reactor.