Mu
sa
shi (M
si) i
s an evolutionarily con
served gene family of RNA-binding protein
s (RBP
s) t
hat i
s preferentially expre
ssed in t
he nervou
s sy
stem. T
he fir
st member of t
he M
si family wa
s identified in
Drosophila.
Drosophila M
si play
s an important role in regulating a
symmetric cell divi
sion of t
he
sen
sory organ precur
sor cell
s. T
he mammalian ort
holog
s, including
human and mou
se Mu
sa
shi1 (M
si1), are neural RBP
s t
hat are
strongly expre
ssed in fetal and adult neural
stem/progenitor cell
s (NS/PC
s). Mammalian M
si1 contribute
s to
self renewal of NS/PC
s t
hroug
h tran
slational regulation of
several target mRNA
s. In t
hi
s study, t
he zebrafi
sh M
si ort
holog
zMsi1 wa
s identified and c
haracterized. T
he normal
spatial and temporal expre
ssion profile
s for bot
h protein and mRNA were determined. A
serie
s of
splice variant
s were detected. Overall, zM
si1 wa
s strongly expre
ssed in neural ti
ssue in early
stage
s of development and ex
hibited
similarity to mammalian M
si1 expre
ssion pattern
s. To reveal t
he
in vivo function of zM
si1, morp
holino
s again
st M
si1 were introduced into one-cell
stage zebrafi
sh embryo
s. Knock down of
zmsi1 frequently re
sulted in aberrant formation of t
he Central Nervou
s Sy
stem (CNS). T
he
se re
sult
s sugge
st t
hat M
si1 play
s role
s in CNS development in vertebrate
s.
This article is part of a Special Issue entitled 鈥淩NA-Binding Proteins鈥?