Leishmania chagasi naturally resistant to nitric oxide isolated from humans and dogs with visceral leishmaniasis in Brazil

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• 作者：P.L. Santos^a ; R.V. Costa^a ; J.M. Braz^a ; L.F.V.C. Santos^a ; A.C. Batista^a ; C.R.O. Vasconcelos^a ; M.R. Rangel^a ; A. Ribeiro de Jesus^a ; ^b ; T.R. de Moura^a ; P.T.G. Leopoldo^a ; R.P. Almeida^a ; ^b ; ^{roquepacheco@uol.com.br} ; ^{plimabio@gmail.com}
• 关键词：HBSS ; Hanks&rsquo ; balanced salt solution ; IC50 ; half maximal inhibitory concentration ; IFN ; interferon ; LPS ; lipopolysaccaride ; VL ; visceral leishmaniasis ; MTT ; 3-metil-[4-5-dimetiltiazol-2-il]-2 ; 5 difeniltetrazó ; lio ; NaNO2 ; sodium nitrite ; NNN ; No
• 刊名：Nitric Oxide
• 出版年：2012
• 期刊代码：181_10898603
• 类别：bio
• 出版时间：30 June, 2012
• 卷：27
• 期：1
• 页码：67-71
• 文件大小：355 K

摘要

Nitric oxide (NO) plays an important role as a leishmanicidal agent in murine macrophages. NO resistant Escherichia coli and Mycobacterium tuberculosis have been associated with poor outcomes of their resulting diseases. NO resistant Leishmania braziliensis has also been identified and exacerbates the clinical course of human leishmaniasis. We report, for the first time, natural resistance of Leishmania chagasi promastigotes to NO. These parasites were isolated from humans and dogs with visceral leishmaniasis. We also demonstrate that this resistance profile was associated with a greater survival capacity and a greater parasite burden in murine macrophages, independent of activation and after activation by IFN-纬 and LPS.