- 作者：Walid Ben-Selma ; wbenselma@hotmail.com ; Hedi Harizi ; Mahmoud Letaief ; Jalel Boukadida
- 关键词：NRAMP1 ; 3&prime ; -UTR ; D543N ; Polymorphism ; Susceptibility ; Tuberculosis
- 刊名：International Journal of Infectious Diseases
- 出版年：2012
- 期刊代码：147_12019712
- 类别：med
- 出版时间：July, 2012
- 卷：16
- 期：7
- 页码：e543-e550
- 文件大小：299 K
Summary
Background
Studies that have assessed NRAMP1 polymorphisms and their association with susceptibility to tuberculosis (TB) in humans have yielded conflicting results. In this study, we evaluated the association between NRAMP1 gene polymorphisms and the risk of the development of active TB in Tunisian populations.Methods
The distribution of 3鈥?UTR and D543N polymorphisms in 223 TB patients (168 patients with pulmonary TB (PTB) and 55 patients with extrapulmonary TB (EPTB)) and 150 healthy donors was determined by PCR-restriction fragment length polymorphism (RFLP) method.
Results
We found that AA and AG genotypes appeared to be associated with susceptibility to PTB (odds ratio (OR) 10.8, 95%confidence interval (CI) 1.37-230.8; p corrected for the number of genotypes (pc) = 0.018) and EPTB (OR 4.37, 95%CI 1.64-11.82; pc = 0.0024), respectively, in patients aged less than 30 years. However, wild-type GG genotype appeared to be associated with resistance against PTB in females (OR 0.1, 95%CI 0.01-0.74; pc = 0.03). The 3鈥?UTR del/del genotype appeared to be associated with susceptibility to PTB in patients aged less than 30 years (OR 3.75, 95%CI 1.5-9.52; pc = 0.003). In contrast, TGTG+/del might be associated with resistance against the development of active PTB (OR 0.23, 95%CI 0.08-0.65; pc = 0.003). A-del haplotype appeared to be associated with susceptibility to PTB (OR 1.79, 95%CI 1.11-2.9; pc = 0.04).
Conclusions
Collectively, our results suggest an association of NRAMP1 3鈥?UTR and D543N polymorphisms with susceptibility to mycobacterial infection in Tunisian populations in relation to age and sex.