Two oral HBx vaccines delivered by live attenuated Salmonella: Both eliciting effective anti-tumor immunity
- 作者：Ye-Jun Wang ; Yan Hou ; He Huang ; Gui-Rong Liu ; Aaron P. White ; Shu-Lin Liu
- 关键词：Tumor vaccine ; Salmonella ; Type III secretion system (TTSS) ; Hepatitis B virus x gene (HBx) ; Anti-tumor immunity
- 刊名：Cancer Letters
- 出版年：2008
- 期刊代码：44_03043835
- 类别：med
- 出版时间：8 May 2008
- 卷：263
- 期：1
- 页码：67-76
- 文件大小：641 K
摘要
Live attenuated bacteria have great potential for use in vaccine development due to several unique advantages, including stable antigen expression, effective antigen presentation, convenient and inexpensive delivery, and low cost of vaccine production. In this study, we expressed hepatitis B virus x gene (HBx) on mouse melanoma cells as the target antigen and constructed Salmonella-based HBx vaccines by two strategies, i.e., recombinant eukaryotic plasmid encoding HBx and a recombinant prokaryotic plasmid encoding Type III secretion system effector-HBx fusion protein. Both HBx constructs elicited significant levels of CTL reaction and IFN-γ secreting T cells. When mice were challenged with melanoma cells expressing HBx, tumor growth rates in immunized animals were significantly slower than controls. Tumor sizes and tumor weight indices of immunized mice were also significantly lower than controls. We conclude that both strategies described in this study may lead to novel approaches of tumor vaccines.