Investigation on the transcription factors of porcine 3尾-hydroxysteroid dehydrogenase and 17尾-hydroxysteroid dehydrogenase genes

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• 作者：Xinxing Dong^a ; Ying Bai^a ; Yi Xin^a ; Qinqin Xu^a ; Gang Chen^b ; ^{chengang02@yahoo.com} ; Meiying Fang^a ; ^{meiying@cau.edu.cn}
• 关键词：Pig ; 3尾-Hydroxysteroid dehydrogenase ; 17尾-Hydroxysteroid dehydrogenase ; Transcription factor
• 刊名：Gene
• 出版年：2012
• 期刊代码：73_03781119
• 类别：bio
• 出版时间：10 May, 2012
• 卷：499
• 期：1
• 页码：186-190
• 文件大小：357 K

摘要

The enzymes 3尾-hydroxysteroid dehydrogenase (3尾HSD) and 17尾-hydroxysteroid dehydrogenase (17尾HSD) regulate the steroid metabolism in mammals. In this study, we aimed to characterize the steroid related transcription factors at the 5鈥?flanking region of these two genes. A series of 5鈥?deletions of approximately 1 kb of 5鈥?flanking region on both genes were fused to a pGL3 basic vector containing firefly luciferase cDNA, and then transfected to human hepatocellular liver carcinoma cell line (HepG2). Luciferase activity assay indicated the region from 鈭?#xA0;574 to 鈭?#xA0;617 bp of the 3尾HSD1 promoter, and from 鈭?#xA0;850 to 鈭?#xA0;868 bp of 17尾HSD7 promoter induced the highest luciferase activity. A putative transcription factor, i.e. the proline and acidic amino acid-rich basic leucine zipper (PAR/bZIP) family of 3尾HSD1 gene, and three-amino acid loop extension (TALE) homeodomain class of 17尾HSD7 were identified respectively by sequence homology. Gel shift assay further confirmed the binding capacity of the putative elements to nuclear extract. Our study gives new insights to the transcriptional regulation of 3尾HSD1 and 17尾HSD7 and further hints to their involvement in steroid metabolism.