Comparative distribution of the mRNAs encoding urotensin I and urotensin II in zebrafish
- 作者：Caroline Parmentier ; Emilie Hameury ; Isabelle Lihrmann ; Jacques Taxi ; Hé ; lè ; ne Hardin-Pouzet ; Hubert Vaudry ; André ; Calas ; Hervé ; Tostivint
- 关键词：Caudal neurosecretory system ; Urotensins ; In situ hybridization
- 刊名：Peptides
- 出版年：2008
- 期刊代码：59_01969781
- 类别：neu
- 出版时间：May 2008
- 卷：29
- 期：5
- 页码：820-829
- 文件大小：1432 K
摘要
The neural neurosecretory system of fishes produces two biologically active neuropeptides, i.e. the corticotropin-releasing hormone paralog urotensin I (UI) and the somatostatin-related peptide urotensin II (UII). In zebrafish, we have recently characterized two UII variants termed UII
and UIIβ. In the present study, we have investigated the distribution of UI, UII and UIIβ mRNAs in different organs by quantitative RT-PCR analysis and the cellular localization of the three mRNAs in the spinal cord by in situ hybridization (ISH) histochemistry. The data show that the UI gene is mainly expressed in the caudal portion of the spinal cord and, to a lesser extent, in the brain, while the UII and the UIIβ genes are exclusively expressed throughout the spinal cord. Single-ISH labeling revealed that UI, UII and UIIβ mRNAs occur in large cells, called Dahlgren cells, located in the ventral part of the caudal spinal cord. Double-ISH staining showed that UI, UII and UIIβ mRNAs occur mainly in distinct cells, even though a few cells were found to co-express the UI and UII genes. The differential expression of UI, UII and UIIβ genes may contribute to the adaptation of Dahlgren cell activity during development and/or in various physiological conditions.
and UIIβ. In the present study, we have investigated the distribution of UI, UII and UIIβ mRNAs in different organs by quantitative RT-PCR analysis and the cellular localization of the three mRNAs in the spinal cord by in situ hybridization (ISH) histochemistry. The data show that the UI gene is mainly expressed in the caudal portion of the spinal cord and, to a lesser extent, in the brain, while the UII and the UIIβ genes are exclusively expressed throughout the spinal cord. Single-ISH labeling revealed that UI, UII and UIIβ mRNAs occur in large cells, called Dahlgren cells, located in the ventral part of the caudal spinal cord. Double-ISH staining showed that UI, UII and UIIβ mRNAs occur mainly in distinct cells, even though a few cells were found to co-express the UI and UII genes. The differential expression of UI, UII and UIIβ genes may contribute to the adaptation of Dahlgren cell activity during development and/or in various physiological conditions.