Vitisin A inhibits adipocyte differentiation through cell cycle arrest in 3T3-L1 cells
- 作者：Soon-hee Kim ; Hee-Sook Park ; Myoung-su Lee ; Yong-Jin Cho ; Young-Sup Kim ; Jin-Taek Hwang ; Mi Jeong Sung ; Myung Sunny Kim ; Dae Young Kwon
- 关键词：Vitisin A ; Adipocyte differentiation ; Cell cycle arrest ; Proliferation
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2008
- 期刊代码：159_0006291x
- 类别：bio
- 出版时间：18 July 2008
- 卷：372
- 期：1
- 页码：108-113
- 文件大小：888 K
摘要
Inhibition of adipocyte differentiation is one approach among the anti-obesity strategies. This study demonstrates that vitisin A, a resveratrol tetramer, inhibits adipocyte differentiation most effectively of 18 stilbenes tested. Fat accumulation and PPARγ expression were decreased by vitisin A in a dose-dependent manner. Vitisin A significantly inhibited preadipocyte proliferation and consequent differentiation within the first 2 days of treatment, indicating that the anti-adipogenic effect of vitisin A was derived from anti-proliferation. Based on cell cycle analysis, vitisin A blocked the cell cycle at the G1-S phase transition, causing cells to remain in the preadipocyte state. Vitisin A increased p21 expression, while the Rb phosphorylation level was reduced. Therefore, vitisin A seems to induce G1 arrest through p21- and consequent Rb-dependent suppression of transcription. On the other hand, ERK and Akt signaling pathways were not involved in the anti-mitotic regulation by vitisin A. Taken together, these results suggest that vitisin A inhibits adipocyte differentiation through preadipocyte cell cycle arrest.