• 作者：Deyanira Estrada-Barraza^a ; Arturo Dá ; valos Martí ; nez^a ; Luis Flores-Padilla^b ; Roberto Mendoza-De Elias^a ; Luis Octavio Sá ; nchez-Vargas^a ; ^{lsanchez@uacj.mx}
• 关键词：Candida ; Reacció ; n en cadena de la polimerasa ; ATB ID32C ; Identificació ; n ; Aislamientos cl&iacute ; nicos
• 刊名：Revista Iberoamericana de Micolog铆a
• 出版年：2011
• 期刊代码：pca320_11301406
• 类别：med
• 出版时间：January-March 2011
• 卷：28
• 期：1
• 页码：36-42
• 文件大小：256 K

The increase in the incidence of yeast species causing fungemia in susceptible immunocompromised patients in the last two decades and the low sensitivity of conventional blood culture has led to the need to develop alternative approaches for the early detection and identification of causative species. The aim of this study was to compare the usefulness of molecular testing by the polymerase chain reaction (PCR) and conventional methods to identify clinical isolates of different species, using the ID32C ATB system (bioM茅rieux, France), chromogenic culture Chromagar Candida庐 (CHROMagar, France) and morphogenesis in corn meal agar. We studied 79 isolates, in which the most prevalent species using the system ID32C and PCR was C. albicans, followed by C. tropicalis, C. glabrata and C .krusei. PCR patterns obtained for the identification of clinical isolates were stable and consistent in the various independent studies and showed good reproducibility, concluding that PCR with species-specific primers that amplify genes ITS1 and ITS2 for rRNA or topoisomerase ii primers is a very specific and sensitive method for the identification of C. glabrata, C. krusei, C. albicans, and with less specificity for C. tropicalis.