Calcium signatures and signaling in cytosol and organelles of tobacco cells induced by plant defense elicitors

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• 作者：Hamid Manzoor^a ; ^b ; ^c ; Annick Chiltz^a ; ^b ; ^c ; Siham Madani^a ; ^b ; ^c ; Parul Vatsa^a ; ^b ; ^c ; ¹ ; Beno&icirc ; t Schoefs^a ; ^b ; ^c ; ² ; Alain Pugin^a ; ^b ; ^c ; Angela Garcia-Brugger^a ; ^b ; ^c ; ^{angela.garcia-brugger@dijon.inra.fr}
• 关键词：Calcium signaling ; Cryptogein ; Oligogalacturonides ; Plant defense ; Chloroplast ; Mitochondria
• 刊名：Cell Calcium
• 出版年：2012
• 期刊代码：7_01434160
• 类别：bio
• 出版时间：June, 2012
• 卷：51
• 期：6
• 页码：434-444
• 文件大小：2025 K

摘要

Calcium signatures induced by two elicitors of plant defense reactions, namely cryptogein and oligogalacturonides, were monitored at the subcellular level, using apoaequorin-transformed Nicotiana tabacum var Xanthi cells, in which the apoaequorin calcium sensor was targeted either to cytosol, mitochondria or chloroplasts. Our study showed that both elicitors induced specific Ca²⁺ signatures in each compartment, with the most striking difference relying on duration. Common properties also emerged from the analysis of Ca²⁺ signatures: both elicitors induced a biphasic cytosolic [Ca²⁺] elevation together with a single mitochondrial [Ca²⁺] elevation concomitant with the first cytosolic [Ca²⁺] peak. In addition, both elicitors induced a chloroplastic [Ca²⁺] elevation peaking later in comparison to cytosolic [Ca²⁺] elevation. In cryptogein-treated cells, pharmacological studies indicated that IP₃ should play an important role in Ca²⁺ signaling contrarily to cADPR or nitric oxide, which have limited or no effect on [Ca²⁺] variations. Our data also showed that, depending on [Ca²⁺] fluxes at the plasma membrane, cryptogein triggered a mitochondrial respiration increase and affected excess energy dissipation mechanisms in chloroplasts. Altogether the results indicate that cryptogein profoundly impacted cell functions at many levels, including organelles.