Insulin-like growth factor 1 (IGF1) and its active peptide (1-3)IGF1 enhance the expression of synaptic markers in neuronal circuits through different cellular mechanisms

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• 作者：Aiden P. Corvin^a ; Ines Molinos^a ; Graham Little^b ; Gary Donohoe^a ; Michael Gill^a ; Derek W. Morris^a ; Daniela Tropea^a ; ^{tropead@tcd.ie}
• 关键词：IGF1 ; insulin-like growth factor 1 ; (1&ndash ; 3)IGF1 ; active peptide of IGF1 (aminoacids 1&ndash ; 3) ; IGF1R ; insulin-like growth factor 1 receptor ; MAPK ; mitogen activated protein kinase ; PI3K ; phosphatidylinositol 3 kinase
• 刊名：Neuroscience Letters
• 出版年：2012
• 期刊代码：52_03043940
• 类别：neu
• 出版时间：27 June, 2012
• 卷：520
• 期：1
• 页码：51-56
• 文件大小：599 K

摘要

Insulin-like growth factor-1 (IGF1) and its active peptide (1-3)IGF1 modulate brain growth and plasticity and are candidate molecules for treatment of brain disorders. IGF1 N-terminal portion is naturally cleaved to generate the tri-peptide (1-3)IGF1 (glycine-praline-glutamate). IGF1 and (1-3)IGF have been proposed as treatment for neuropathologies, yet their effect on nerve cells has not been directly compared. In this study we examine the effects of IGF1 and (1-3)IGF1 in primary cortical cultures and measure the expression levels of markers for intracellular pathways and synaptic function. We find that both treatments activate the IGF1 receptor and enhance the expression of synaptic markers, however, they activate different intracellular pathways. Furthermore, (1-3)IGF1 administration increases the expression of endogenous IGF1, suggesting a direct interaction between the two molecules. The results show that the two molecules increase the expression of synaptic proteins through activating different cellular mechanisms.