House dust bioactivities predict skin prick test reactivity for children with high risk of allergy

详细信息	查看全文 | 推荐本文 |

• 作者：Haejin Kim ; MD^a ; Kevin Tse ; MD^b ; Linda Levin ; PhD^c ; David Bernstein ; MD^a ; ^c ; Tiina Reponen ; PhD^c ; Grace LeMasters ; PhD^c ; Zana Lummus ; PhD^a ; Anthony A. Horner ; MD^b ; ^d ; ^{ahorner@ucsd.edu}
• 关键词：House dust extract ; aeroallergen sensitization ; tolerance ; allergy ; skin prick test ; human ; innate immunity
• 刊名：Journal of Allergy and Clinical Immunology
• 出版年：2012
• 期刊代码：158_00916749
• 类别：med
• 出版时间：June, 2012
• 卷：129
• 期：6
• 页码：1529-1537.e2
• 文件大小：429 K

摘要

| Figures/TablesFigures/Tables | ReferencesReferences

Background

Although evidence suggests that ambient exposures to endotoxin and other immunostimulants during early life influence allergic risk, efforts to understand this host-environment relationship have been hampered by a paucity of relevant assays.

Objectives

These investigations determined whether parameters of house dust extract (HDE) bioactivity were predictive of allergen skin prick test (SPT) reactivity for infants at high risk of allergy participating in the Cincinnati Childhood Allergy and Air Pollution Study (CCAAPS).

Methods

We conducted a nested case-control study, selecting 99 CCAAPS children who had positive SPT results to at least 1 aeroallergen at age 3 years and 101 subjects with negative SPT results. HDEs were prepared from dust samples collected from the subjects鈥?homes at age 1 year. Murine splenocytes and bone marrow-derived dendritic cells were incubated with HDEs, and supernatant cytokine concentrations were determined by means of ELISA. Alternatively, bone marrow-derived dendritic cells were preincubated with HDEs, and then LPS-induced IL-6 responses were assessed. HDE endotoxin levels were determined by using the limulus amebocyte lysate assay.

Results

HDEs derived from the homes of children with positive (cases) and negative (control subjects) SPT results had similar bioactivities. However, when cases were considered in isolation, HDEs with higher levels of bioactivity were significantly associated with children who had lower numbers of positive SPT results. Analogous statistical analyses did not identify any association between HDE endotoxin levels and the aeroallergen sensitization profiles of children included in this study.

Conclusion

HDE immunostimulatory activities predicted the aeroallergen sensitization status of CCAAPS subjects better than HDE endotoxin levels. These results provide the first published evidence that HDE bioassays have clinical relevance in predicting atopic risk.