Alterations of profibrinolytic receptor annexin A2 in pre-eclampsia: A possible role in placental thrombin formation

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• 作者：Hong Xin^a ; Yi Zhang^b ; Huilan Wang^a ; ^{xh.hbyd@hotmail.com} ; Shuhan Sun^b ; ^{shsun@vip.sina.com}
• 关键词：PE ; pre-eclampsia ; tPA ; tissue-type plasminogen activator ; uPA ; urokinase-type plasminogen activator ; PAIs ; plasminogen activator inhibitors ; APS ; antiphospholipid syndrome ; ELISA ; enzyme-linked immunosorbent assay ; IUGR ; intrauterine growth restriction
• 刊名：Thrombosis Research
• 出版年：2012
• 期刊代码：247_00493848
• 类别：med
• 出版时间：May, 2012
• 卷：129
• 期：5
• 页码：563-567
• 文件大小：888 K

摘要

Introduction

Deficient fibrinolytic activity due to alterations of profibrinolytic receptor annexin A2 has been shown to be related to increased thrombosis. We compared the expression of annexin A2 in pre-eclampsia with that in normal pregnancies and investigated its relationship to placental thrombin formation.

Materials and methods

Sixty patients with pre-eclampsia and 30 matched normal pregnant controls were included in the study. Expression of annexin A2 mRNA in placental tissues was analyzed using quantitative RT-PCR. Annexin A2 protein in placentas as well as blood was determined by western blot analysis and immunohistochemistry. Enzyme-linked immunosorbent assay (ELISA) was used to detect the presence of anti- annexin A2 antibodies in peripheral maternal blood.

Results

The expression of annexin A2 mRNA in placentas was significantly decreased in pre-eclampsia compared to normal pregnancies. Levels of annexin A2 protein in placentas as well as in peripheral maternal blood were also significantly reduced in patients with pre-eclampsia compared with healthy pregnant women. High titers of anti- annexin A2 antibodies were more frequently detected in pre-eclampsia group. Decreased levels of annexin A2 expression and the presence of anti-annexin A2 antibodies were associated with increased placental thrombosis.

Conclusion

The down regulation of annexin A2 expression and the presence of anti-annexin A2 antibodies were correlated to PE and may increase placental thrombin formation.