Description of an Atlantic salmon (Salmo salar L.) type II interleukin-1 receptor cDNA and analysis of interleukin-1 receptor expression in amoebic gill disease-affected fish
- 作者：R.N. Morrison ; Richard.Morrison@dpipwe.tas.gov.au ; N.D. Young1 ; youngnd@unimelb.edu.au ; B.F. Nowak
- 关键词：Interleukin-1beta ; IL-1 receptor ; Amoebic gill disease
- 刊名：Fish & Shellfish Immunology
- 出版年：2012
- 期刊代码：115_10504648
- 类别：abs
- 出版时间：June, 2012
- 卷：32
- 期：6
- 页码：1185-1190
- 文件大小：976 K
摘要
Previously, we showed that IL-1尾 transcription is induced in the gills of amoebic gill disease (AGD)-affected fish in an AGD lesion-restricted fashion. However, in this environment, there is very little evidence of inflammation on histopathological or transcriptional levels and we hypothesised that aberrant signalling may occur. As a first step in investigating this issue, we cloned and sequenced the Atlantic salmon IL-1 receptor type II (IL-1RII) mRNA, and then examined the expression of both the IL-1RI (IL-1 receptor-like protein) and II during Neoparamoeba perurans infection. In gill lesions from AGD-affected fish, a step-wise temporal increase in the relative expression of IL-1尾 coincided with a significant reduction in IL-1RI, whereas the IL-1RII mRNA remained unchanged. Down-regulation of IL-1RI could explain the paucity of inflammation in affected tissue, although simultaneous up-regulation of IL-1尾-inducible transcripts indicated that this is not due to a complete blockage of the IL-1RI pathway. Rather, it appears that IL-1RI transcription is reduced and this rate limits the effects of chronic IL-1尾 over-expression.