Molecular characterization, tissue distribution and expression analysis of Interleukin-12 receptor 尾2 chain in sheep

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• 作者：Xuelian Meng¹ ; Aijiang Guo¹ ; Wei Gong ; Wanzhong Jia ; Xuenong Luo ; Junjun Zhai ; Yongxi Dou ; Xuepeng Cai ; ^{caixp@vip.163.com}
• 关键词：APC ; antigen-presenting cells ; CTL ; cytotoxic lymphocytes ; FN3 ; fibronectin type 3 ; IL-12 ; Interleukin-12 ; IL-12R ; interleukin (IL)-12 receptor ; IL-12R尾1 ; interleukin (IL)-12 receptor beta 1 ; IL-12R尾2 ; interleukin (IL)-12 receptor beta 2 ; IPTG ; isopropy
• 刊名：Gene
• 出版年：2012
• 期刊代码：73_03781119
• 类别：bio
• 出版时间：10 May, 2012
• 卷：499
• 期：1
• 页码：124-129
• 文件大小：1288 K

摘要

Ovine 尾2 subunit of the interleukin (IL)-12 receptor (IL-12R尾2) was cloned from mRNA preparation of mitogen-activated peripheral blood mononuclear cells (PBMCs). The complete coding sequence for ovine IL-12 R尾2 was found to be 2586 nucleotides in length encoding 862-amino-acid residue protein. It showed 96.4%homology at the nucleotide level and 94.1%homology at the amino acid level with bovine IL-12 R尾2. The ovine IL-12 R尾2 subunit shares common structural and functional elements with their counterparts from the other species. Phylogenetic tree showed that ovine IL-12R尾2 was clustered into the Artiodactyla group, together with those of cattle and pig, which was distinct from the other groups. Real-time RT-PCR was used to investigate expression of the IL-12R尾2 in different tissues of sheep in order to determine the characterization of this receptor in tissue. Expression analysis showed that IL-12R尾2 mRNA expression was detected at all the detected tissues with the exception of thymus.