Platelet proteome and clopidogrel response in patients with stable angina undergoing percutaneous coronary intervention

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• 作者：Elisabetta Volpi^a ; ^{volpi@ifc.cnr.it} ; Laura Giusti^b ; ^{giusti@farm.unipi.it} ; Federica Ciregia^b ; ^{ciregia@farm.unipi.it} ; Ylenia Da Valle^b ; ^{ylenia.davalle@gmail.com} ; Gino Giannaccini^b ; ^{ggino@farm.unipi.it} ; Sergio Berti^c ; ^{sergio.berti@ftgm.it} ; Aldo Clerico^a ; ^{clerico@ifc.cnr.it} ; Antonio Lucacchini^b ; ^{lucas@farm.unipi.it}
• 关键词：PCI ; percutaneous coronary intervention ; 2DE ; two-dimensional gel electrophoresis ; NSTE-ACS ; non-ST segment elevation acute coronary syndrome ; NSAID ; non-steroidal anti-inflammatory drugs ; COX-2 ; cyclooxygenase 2 ; PRP ; Platelet-rich plasma ; FITC ; fluoresc
• 刊名：Clinical Biochemistry
• 出版年：2012
• 期刊代码：55_00099120
• 类别：med
• 出版时间：July, 2012
• 卷：45
• 期：10-11
• 页码：758-765
• 文件大小：686 K

摘要

Objectives

We analyzed the platelet proteome of circulating platelets during the onset of clopidogrel therapy in patients with stable angina underwent percutaneous coronary intervention in order to investigate the mechanisms that control platelet reactivity and clopidogrel response in this context.

Design & methods

Twenty patients were enrolled in this study. Blood samples were collected before coronary angiography (T0), 12 h after 600 mg of clopidogrel (T1) and 24 h after percutaneous coronary intervention (PCI) (T2). Platelet reactivity, Clopidogrel response and proteomic analysis were examined.

Results

Clopidogrel loading dose produced a significant inhibition in all markers of platelet activation in both flow cytometry and aggregation tests. Among the proteins found differentially expressed, eighteen were identified by MS/MS analysis and they resulted involved in the cytoskeleton rearrangement (profilin-1, calpain, 伪-soluble NSF attachment protein, thrombospondin), in the energetic metabolism (ubiquitin-like modifier-activating enzyme 1, protein-L-isoaspartate-(D-aspartate) O-methyltransferase and nucleoside diphosphate kinase B) and in the oxidative stress (heat shock 70 kDa protein 5 and anti-stress induced phosphoprotein 1.

Conclusions

The present study provides novel information on platelet proteome changes associated with platelet activation and clopidogrel response. This investigation supports the development of further proteomic studies for the identification of novel platelet biomarkers.