Role of radiation-induced granulocyte colony-stimulating factor in recovery from whole body gamma-irradiation

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• 作者：Vijay K. Singh^a ; ^b ; ^{singh@afrri.usuhs.mil} ; Oluseyi O. Fatanmi^a ; Pankaj K. Singh^a ; Mark H. Whitnall^a
• 关键词：Cytokines ; G-CSF ; Immunohistochemistry ; Mice ; Neutralization ; Radiation
• 刊名：Cytokine
• 出版年：2012
• 期刊代码：97_10434666
• 类别：imm
• 出版时间：June, 2012
• 卷：58
• 期：3
• 页码：406-414
• 文件大小：909 K

摘要

The purpose of this study was to further elucidate the radioprotective role of granulocyte colony-stimulating factor (G-CSF) induced in response to irradiation. The induction of G-CSF and interleukin-6 (IL-6) in response to radiation exposure was evaluated in mice. The level of cytokine in serum was determined by multiplex Luminex. The role of G-CSF on survival and tissue injury after total body gamma-irradiation was evaluated by administration of neutralizing antibody to G-CSF before radiation exposure. An isotype control was used for comparison and survival was monitored for 30 d after irradiation. Jejunum samples were used for immunohistochemistry. Ionizing radiation exposure induced significant levels of the hematopoietic cytokines G-CSF and IL-6, in mice receiving 9.2 Gy radiation. Maximal levels of G-CSF were observed in peripheral blood of mice 8 h after irradiation. IL-6 levels were maximum at 12 h after irradiation. Administration of G-CSF antibody significantly enhanced mortality in irradiated mice. G-CSF antibody-treated mice had higher numbers of CD68⁺ cells and apoptotic cells in intestinal villi. Our results confirm that radiation exposure induces elevations of circulating G-CSF and IL-6. Neutralizing antibody to G-CSF exacerbates the deleterious effects of radiation, indicating that G-CSF induced in response to irradiation plays an important role in recovery.