Statistical evaluation of the isoform patterns of N-acetyl-β-hexosaminidase from human renal cancer tissue separated by isoelectrofocusing

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• 作者：Malgorzata Borzym-Kluczyk ; Iwona Radziejewska ; Ewa Olszewska ; Sł ; awomir Szajda ; Mał ; gorzata Knaś ; ; Krzysztof Zwierz
• 关键词：HEX A and B ; Isoforms ; N-acetyl-β ; -glucosaminidase ; Renal cancer
• 刊名：Clinical Biochemistry
• 出版年：2007
• 期刊代码：55_00099120
• 类别：med
• 出版时间：March 2007
• 卷：40
• 期：5-6
• 页码：403-406
• 文件大小：483 K

摘要

>Objectives

Isoenzymes of HEX from human renal carcinoma and neighbouring macroscopically normal renal tissue can show different patterns on isoelectrofocusing gels. The aim of our work was to elaborate the method for statistical evaluation of differences.

Design and methods

Isoenzymes of N-acetyl-β-hexosaminidase were separated from renal (control and cancerous) tissues of 15 patients. Isoenzymes were electrofocused in Multiphor II, with ampholine pH 3.5–9.0 (2%) and then evaluated densitometrically and analysed statistically.

Results

A similar pattern in activity of isoforms of isoenzymes A and B in normal and cancerous renal tissue was observed.

Conclusions

The proposed method of statistical evaluation of differences in isoforms of N-acetyl-β-glucosaminidase can also be adapted to estimate the isoforms of other enzymes in different tissues.