Identification of new altered genes in rat cochleae with noise-induced hearing loss

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• 作者：Yu Han^a ; ¹ ; Liu Hong^b ; ¹ ; Cuiping Zhong^c ; ¹ ; Yang Chen^a ; Ye Wang^a ; Xiaobo Mao^a ; Daqing Zhao^a ; Jianhua Qiu^a ; ^{qiujh@fmmu.edu.cn}
• 关键词：qRT-PCR ; Quantitative realtime polymerase chain reaction ; NIHL ; noise induced hearing loss ; ABR ; Auditory brainstem responses ; OHCs ; outer hair cells ; IHCs ; inner hair cells ; EASE ; Expression Analysis Systematic Explorer ; SD ; standard deviation
• 刊名：Gene
• 出版年：2012
• 期刊代码：73_03781119
• 类别：bio
• 出版时间：15 May, 2012
• 卷：499
• 期：2
• 页码：318-322
• 文件大小：379 K

摘要

Because genes that are highly expressed in the cochlea after noise stress may have crucial regulatory roles in hearing, the identification of these genes may be useful for restoring normal auditory function. This study assessed altered gene expression at 1 h following the cessation of noise exposure by using microarrays and real-time polymerase chain reaction (qPCR) in rats. In addition, the auditory threshold shifts and morphological changes of hair cells were observed. This study indicated that applied noise induced outer hair cell loss and a 40-50 dB hearing loss. Totally 239 altered genes were involved in the immune system process, response to stress, or response to stimulus. The expression of five up-regulated genes (Reg3b, Lcn2, Serpina3n, Nob1 and Hamp) was confirmed by qPCR. Future experiments will focus on several of these new candidate genes and may provide insight into the underlying auditory pathophysiology.