Protective effects of ginsenoside Rb1, ginsenoside Rg1, and notoginsenoside R1 on lipopolysaccharide-induced microcirculatory disturbance in rat mesentery
- 作者：Kai Sun ; Chuan-She Wang ; Jun Guo ; Yoshinori Horie ; Su-Ping Fang ; Fang Wang ; Yu-Ying Liu ; Lian-Yi Liu ; Ji-Ying Yang ; Jing-Yu Fan ; Jing-Yan Han
- 关键词：Panax notoginseng ; Microcirculatory disturbance ; Leukocyte adhesion molecules ; Hydrogen peroxide ; Mast cell degranulation ; Lipopolysaccharide
- 刊名：Life Sciences
- 出版年：2007
- 期刊代码：62_00243205
- 类别：imm
- 出版时间：19 July 2007
- 卷：81
- 期：6
- 页码：509-518
- 文件大小：648 K
摘要
Ginsenoside Rb1 (Rb1), ginsenoside Rg1 (Rg1), and notoginsenoside R1 (R1) are major active components of Panax notoginseng, a Chinese herb that is widely used in traditional Chinese medicine to enhance blood circulation and dissipate blood stasis. To evaluate the effect of these saponins on microcirculatory disturbance induced by lipopolysaccharide (LPS), vascular hemodynamics in rat mesentery was observed continuously during their administration using an inverted microscope and a high speed video camera system. LPS administration decreased red blood cell velocity but Rb1, Rg1, and R1 attenuated this effect. LPS administration caused leukocyte adhesion to the venular wall, mast cell degranulation, and the release of cytokines. Rb1, Rg1, and R1 reduced the number of adherent leukocytes, and inhibited mast cell degranulation and cytokine elevation. In vitro experiments using flow cytometry further demonstrated that a) the LPS-enhanced expression of CD11b/CD18 by neutrophils was significantly depressed by Rb1 and R1, and b) hydrogen peroxide (H2O2) release from neutrophils in response to LPS stimulation was inhibited by treatment with Rg1 and R1. These results suggest that the protective effect of Rb1 and R1 against leukocyte adhesion elicited by LPS may be associated with their suppressive action on the expression of CD11b/CD18 by neutrophils. The protective effect against mast cell degranulation by Rb1 and R1, and the blunting of H2O2 release from neutrophils by Rg1 and R1 suggest mechanistic diversity in the effects of Panax notoginseng saponins in the attenuation of microcirculatory disturbance induced by LPS.