A rapid and sensitive LC/ESI-MS/MS method for quantitative analysis of docetaxel in human plasma and its application to a pharmacokinetic study

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• 作者：Hiroaki Yamaguchi^a ; Asuka Fujikawa^a ; Hajime Ito^a ; Nobuaki Tanaka^a ; Ayako Furugen^a ; Kazuaki Miyamori^a ; Natsuko Takahashi^b ; Jiro Ogura^a ; Masaki Kobayashi^a ; Takehiro Yamada^c ; Nariyasu Mano^d ; Ken Iseki^a ; ^{ken-i@pharm.hokudai.ac.jp}
• 关键词：LC/MS/MS ; liquid chromatography/tandem mass spectrometry ; LLOQ ; lower limit of quantification ; MRM ; multiple reaction monitoring
• 刊名：Journal of Chromatography B ; Analytical Technologies in the Biomedical and Life Sciences
• 出版年：2012
• 期刊代码：124_15700232
• 类别：ch
• 出版时间：15 April, 2012
• 卷：893-894
• 期：Complete
• 页码：157-161
• 文件大小：419 K

摘要

Docetaxel is a taxane family antineoplastic agent widely employed in cancer chemotherapy. We developed a liquid chromatography/tandem mass spectrometry method for the determination of docetaxel in human plasma. Plasma samples were deproteinized by acetonitrile containing internal standard paclitaxel. Chromatographic separation was performed on a TSKgel ODS-100V 3 渭m (50 mm 脳 2.0 mm i.d.) column using a mobile phase composed of acetonitrile-methanol-water-formic acid (50:5:45:0.1, v/v/v/v). Detection was performed on a triple-quadrupole tandem mass spectrometer with multiple reaction monitoring (MRM) mode via electrospray ionization (ESI) source. This method covered a linearity range of 5-5000 ng/mL with the lower limit of quantification of 5 ng/mL. The intra-day precision and inter-day precision (R.S.D.) of analysis were less than 6.7%, and the accuracy (R.E.) was within 卤9.0%at the concentrations of 5, 20, 200, and 2000 ng/mL. The total run time was 5.0 min. This method was successfully applied for clinical pharmacokinetic investigation.