In vivo efficacy of a novel liposomal formulation of safingol in the treatment of acute myeloid leukemia

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• 作者：Kuan-Boone Tan^a ; Leong-Uung Ling^a ; Ralph M. Bunte^b ; Wee-Joo Chng^c ; ^d ; ^e ; Gigi N.C. Chiu^a ; ^{phacncg@nus.edu.sg}
• 关键词：Acute myeloid leukemia ; Liposome ; Safingol ; Sphingolipid ; Zeta potential ; Hemolysis
• 刊名：Journal of Controlled Release
• 出版年：2012
• 期刊代码：25_01683659
• 类别：pha
• 出版时间：10 June, 2012
• 卷：160
• 期：2
• 页码：290-298
• 文件大小：860 K

摘要

Prognosis of patients with acute myeloid leukemia (AML) remains poor despite the use of first-line induction chemotherapy. Therefore, it is imperative to find effective treatment for AML patients. Safingol is a bioactive sphingolipid which has demonstrated promising in vitro anti-leukemic properties; however, translation into clinical use is hampered by its low water solubility and dose-limiting hemolysis. The present study is the first to describe a rationally designed liposome formulation of safingol and demonstrate the anti-leukemic potential using a panel of human AML cell lines and patient samples as well as a human xenograft model in SCID mice. Encapsulation efficiency of safingol into liposomes was approximately 100%, and the release of drug followed square-root-of-time release model. The presence of a transmembrane pH gradient completely abolished the biological activity of liposomal safingol. A positive zeta potential, which influenced cellular accumulation of liposomal safingol, was crucial to the anti-leukemic activity. Liposomal safingol was effective against a wide range of AML subtypes with minimal hemolytic toxicity, and was able to extend the median survival time of the U937-inoculated mice to 31 days as compared to 23 days by free drug. The increase in therapeutic efficacy could be related to the increase in systemic drug exposure as a result of liposome encapsulation.