A novel on-line gold nanoparticle-catalyzed luminol chemiluminescence detector for high-performance liquid chromatography

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• 作者：Qun Lin Zhang ; ^{qlzhang@ahmu.edu.cn} ; Liang Wu ; Chen Lv ; Xiao Yue Zhang
• 关键词：On-line ; Gold nanoparticle ; Chemiluminescence ; High-performance liquid chromatography ; Luminol ; Phenolic compound
• 刊名：Journal of Chromatography A
• 出版年：2012
• 期刊代码：47_00219673
• 类别：ch
• 出版时间：15 June, 2012
• 卷：1242
• 期：Complete
• 页码：84-91
• 文件大小：1056 K

摘要

A novel on-line gold nanoparticle-catalyzed luminol-H₂O₂ chemiluminescence (CL) detector for high-performance liquid chromatography (HPLC) was established, in which gold nanoparticles were produced by the on-line reaction of H₂O₂, NaHCO₃-Na₂CO₃ (buffer solution of luminol), and HAuCl₄. Eight phenolic compounds (gallic acid, protocatechuic acid, protocatechuic aldehyde, 2,5-dihydroxybenzoic acid, caffeic acid, 2,3-dihydroxybenzoic acid, (+)-catechin, and (鈭?-epicatechin) were chosen as the model compounds. Every separated phenolic compound in the column eluent strongly enhanced the CL signal of on-line gold nanoparticle-catalyzed luminol system. The CL and UV-visible absorption spectra and transmission electron microscopy studies were carried out, and the CL enhancement mechanism was ascribed to that the presence of phenolic compound promoted the on-line formation of 38-nm-diameter gold nanoparticles, which better catalyzed the luminol-H₂O₂ CL reaction. The effects of methanol and phosphoric acid in the proposed HPLC configuration were performed by two gradient elution programs, and the baseline profile revealed that on-line gold nanoparticle-catalyzed luminol-H₂O₂ CL detector had better compatibility than 38 nm gold colloids-luminol-H₂O₂ CL detector. The proposed CL detector exhibits excellent analytical performance with the low detection limit (S/N = 3) of 0.53-0.97 ng/mL (10.6-19.4 pg) phenolic compounds, and offers a new strategy for developing on-line nanoparticle-catalyzed CL detector for HPLC with sensitive analysis.