Arginase I and the very low-density lipoprotein receptor are associated with phenotypic biomarkers for obesity

详细信息	查看全文 | 推荐本文 |

• 作者：Oh Yoen Kim ; Ph.D.^a ; Seung-Min Lee ; Ph.D.^b ; Ji Hyung Chung ; Ph.D.^a ; ^c ; Hyun Joo Do ; M.S.^b ; Jiyoung Moon ; M.S.^b ; Min-Jeong Shin ; Ph.D.^b ; ^{mjshin@korea.ac.kr}
• 关键词：Obesity ; Arginase I ; Very low-density lipoprotein receptor ; Soluble intercellular adhesion molecule-1 ; Cardiovascular risk
• 刊名：Nutrition
• 出版年：2012
• 期刊代码：81_08999007
• 类别：abs
• 出版时间：June, 2012
• 卷：28
• 期：6
• 页码：635-639
• 文件大小：167 K

摘要

Objective

Obesity is a serious health problem implicated in many metabolic disorders (i.e., hypertension, dyslipidemia, and cardiovascular disease). We examined whether the mRNA of tested genes were linked to blood lipid concentrations and vascular endothelial function as features of obesity.

Methods

In healthy subjects (30-69 y old; normal weight, n = 22, body mass index 18.5-23 kg/m²; overweight, n = 25, body mass index 鈮?3 kg/m²) the following parameters were measured in the blood circulation: total cholesterol, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, triacylglycerol, apolipoprotein AI, apolipoprotein B, adiponectin, high-sensitivity C-reactive protein, and soluble intercellular adhesion molecule-1. The mRNA levels of genes (arginase I [ARG1], very low-density lipoprotein receptor [VLDLR], adiponectin receptor-1 [ADIPOR1], ADIPOR2, and nitric oxide synthase-3 [NOS3]) were tested in the subjects鈥?peripheral blood mononuclear cells.

Results

The expression levels of all tested genes were investigated for their associations with the blood concentrations of each parameter. In the expression study, only ARG1 (4.5-fold) and VLDLR (4-fold) expressions were significantly upregulated in the overweight group compared with the normal-weight group. The ARG1 mRNA levels were positively associated with blood concentrations of total cholesterol, low-density lipoprotein cholesterol, apolipoprotein B, and soluble intercellular adhesion molecule-1. The VLDLR mRNA levels showed a positive relation with triacylglycerol and glucose concentrations and a negative relation with adiponectin levels.

Conclusion

Significant upregulations of ARG1 and VLDLR were observed in the overweight condition and their expression levels are likely to be closely linked to the phenotypic biomarkers for obesity (disturbed lipid profiles and endothelial dysfunction).