Liuwei Dihuang decoction facilitates the induction of long-term potentiation (LTP) in senescence accelerated mouse/prone 8 (SAMP8) hippocampal slices by inhibiting voltage-dependent calcium channels (VDCCs) and promoting N-methyl-d-aspartate receptor (NMD

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• 作者：Yan Huang¹ ; Heping Zhang¹ ; Sheng Yang ; Haifa Qiao ; Wenxia Zhou ; ^{zhouwx@nic.bmi.ac.cn} ; Yongxiang Zhang ; ^{zhangyx@nic.bmi.ac.cn}
• 关键词：N-methyl-d-aspartate receptor (NMDA) receptor ; Voltage-dependent calcium channel (VDCC) ; Long-term potentiation (LTP) ; Liuwei Dihuang Decotion (LW) ; Senescence accelerated mouse/prone 8 (SAMP8)
• 刊名：Journal of Ethnopharmacology
• 出版年：2012
• 期刊代码：26_03788741
• 类别：pha
• 出版时间：27 March, 2012
• 卷：140
• 期：2
• 页码：384-390
• 文件大小：689 K

摘要

Ethnopharmacological relevance

Liuwei Dihuang decoction (LW) is a typical traditional Chinese medicine (TCM) prescription and consists of six herbs including Rehmannia glutinosa Libosch. (family: Scrophulariaceae), Cornus officinalis Sieb. (family: Cornaceae), Dioscorea opposite Thunb. (family: Dioscoreaceae), Alisma orientale (G. Samuelsson) Juz (family: Alismataceae), Poria cocos (Schw.)Wolf (family: Polyporaceae) and Paeonia suffruticosa Andrews (family: Paeoniaceae). It has long been used clinically in treatment of many kinds of diseases with the sign of Yin insufficiency of kidney.

Aim of the study

Our previous pharmacological studies demonstrated that LW possesses effect of ameliorating the decline of the learning and memory in senescence accelerated mouse/prone 8 (SAMP8), but the mechanism has not been well established. LW-containing serum (LWCS) is used in the current study to elucidate the possible mechanisms.

Materials and methods

6-month-old SAMP8 was used in this study to investigate the effect of LWCS on the induction of long-term potentiation (LTP). Primary cultured hippocampal neurons were used in this study to investigate the effects of LWCS on [Ca²⁺]_i, I_Ca and N-Methyl-d-aspartate (NMDA)-evoked currents. [Ca²⁺]_i was imaged using Fluo-3 and whole-cell patch recordings were applied to study the I_Ca and NMDA-evoked currents.

Results

We find that LWCS facilitates the induction of LTP in hippocampal slices of 6-month-old SAMP8. In primary cultured hippocampal neurons, LWCS increases intracellular [Ca²⁺]_i, the I_Ca is suppressed by LWCS, and NMDA-evoked currents are promoted.

Conclusion

These results indicate that LW improves the synaptic plasticity by inhibiting voltage-dependent calciumchannels (VDCCs) and promoting the function of NMDA receptors. This improvement might be one of the mechanisms contributing to cognitive improvement effect of LW.