Loss of tumorigenicity of human pancreatic carcinoma cells engineered to produce interleukin-2 or interleukin-4 in nude mice: a potentiality for cancer gene therapy
- 作者：Kimura ; Masaki ; Tagawa ; Masatoshi ; Takenaga ; Keizo ; Kondo ; Fukuo ; Yamaguchi ; Taketo ; Saisho ; Hiromitsu ; et. al.
- 关键词：Pancreatic cancer ; Interleukin-2 ; Interleukin-4 ; Gene therapy ; AsPC-1
- 刊名：Cancer Letters
- 出版年：1998
- 期刊代码：44_03043835
- 类别：med
- 出版时间：June 5, 1998
- 卷：128
- 期：1
- 页码：47-53
- 文件大小：308 K
摘要
To examine the possibility of cytokine gene therapy in relation to pancreatic cancer, we evaluated the antitumor effect of human pancreatic carcinoma cells (AsPC-1) which were retrovirally-transduced with several kinds of cytokine genes. These cells were inoculated into BALB/c nude mice and their tumor volumes were assessed. The in vitro growth rate of the transduced cells was not different from that of a parental cell line. Among the transduced cells, human interleukin (IL)-6-transduced AsPC-1 and mouse granulocyte macrophage colony-stimulating factor-transduced AsPC-1 cells showed a significant retardation of tumor growth compared with a parental cell line. In the cases of AsPC-1 cells transduced with the human IL-2 or mouse IL-4 gene, small tumors were generated but thereafter they regressed completely. Histological examinations showed monocytic cell infiltration around the tumors of IL-2- or IL-4-producing cells. These data suggest that secretion of IL-2 or IL-4 from tumor cells can induce an antitumor effect even in the defective condition of mature T cells.