Polyunsaturated fatty acid supplements modulate mast cell membrane microdomain composition

详细信息	查看全文 | 推荐本文 |

• 作者：Shereen Basiouni^a ; ^b ; Katja Stö ; ckel^a ; Herbert Fuhrmann^a ; Julia Schumann^a ; ^{julia.schumann@vmf.uni-leipzig.de}
• 关键词：Lipid rafts ; Mast cells ; Membrane fatty acid profiles ; PUFA
• 刊名：Cellular Immunology
• 出版年：2012
• 期刊代码：279_00088749
• 类别：med
• 出版时间：January-February, 2012
• 卷：275
• 期：1-2
• 页码：42-46
• 文件大小：279 K

摘要

In the present study, the lipid raft composition of a canine mastocytoma cell line (C2) was analyzed. Lipid rafts were well separated from non-raft plasma membranes using a detergent-free isolation technique. To study the influence of n-3 and n-6 polyunsaturated fatty acids (PUFA) on raft fatty acid composition in comparison to non-raft cell membrane, C2 were supplemented with one of the following: 伪-linolenic acid, eicosapentaenoic acid, docosahexaenoic acid, linoleic acid or arachidonic acid. Enrichment of the culture medium with a specific PUFA resulted in an increase in the content of this fatty acid both in rafts and non-raft membranes. Contents of cholesterol and protein were found not to be affected by the changes in the fatty acid profiles. In conclusion, our data provide strong evidence that PUFA modulate lipid composition and physiological properties of membrane micro domains of mast cells which in turn may have effects on mast cell function.