A comparison between neurally induced bone marrow derived mesenchymal stem cells and olfactory ensheathing glial cells to repair spinal cord injuries in rat

详细信息	查看全文 | 推荐本文 |

• 作者：Saeed Oraee Yazdani^a ; ^b ; ^c ; Mirsepehr Pedram^d ; Maryam Hafizi^b ; Mahboubeh Kabiri^b ; ^f ; Masoud Soleimani^e ; ^{Soleim_m@modares.ac.ir} ; Mohamad-Mehdi Dehghan^d ; Issa Jahanzad^g ; Yousof Gheisari^b ; Seyed Mahmoud Hashemi^b ; ^h
• 关键词：Spinal cord injury ; Neurally induced mesenchymal stem cell ; Olfactory ensheathing cell ; Balloon induced compression
• 刊名：Tissue and Cell
• 出版年：2012
• 期刊代码：21_00408166
• 类别：abs
• 出版时间：August, 2012
• 卷：44
• 期：4
• 页码：205-213
• 文件大小：2105 K

摘要

Cell therapy has proven to be a highly promising method in clinical applications, raising so much hope for the treatment of injured tissues with low, if any, self regeneration potential such as central and peripheral nervous system. Neurally induced bone marrow derived mesenchymal stem cells (NIMSCs) as well as olfactory ensheathing cells (OECs) were transplanted in a rat model of sub-acute spinal cord injury and the behavioral and histological analyses were conducted. A balloon-compression technique was used to produce an injury at T8-T9 level of spinal cord. After a week post injury, rats were injected with either NIMSCs or OECs at the center of developing lesion cavity, 3 mm cranial and 3 mm caudal to the cavity. Weekly behavioral assessment using BBB score was done over five-week period post transplantation and finally histological assessment was performed to locate labeled cells in the tissue in order to evaluate the reduction of cavity formation and axonal regeneration. Evaluation of locomotor performance showed significant behavioral improvement in NIMSC group over OEC and control groups. The histological analyses revealed the presence of transplanted cells in the spinal cord parenchyma. Volume of injured area that was occupied with syrinx cavity in NIMSC group was significantly less than control group. In addition, meanwhile neurofilament-positive axons significantly showed higher expression in rats receiving NIMSC compared to the other two groups. In conclusion NIMSC caused both behavioral and histological improvement that potentially makes them a promising candidate for cell therapy approaches of spinal cord injuries.