Metalloproteinases 2 and 9 and their tissue inhibitors 1 and 2 are increased in vulvar lichen sclerosus

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• 作者：Guilherme A.P. de Oliveira^a ; ¹ ; Mô ; nica P. de Almeida^b ; ^c ; ¹ ; Fernando A. Soares^d ; Gutemberg L. de Almeida Filho^e ; Christina M. Takiya^b ; Ivone B. Otazu^f ; Luiz E. Nasciutti^b ; ^{luiz.nasciutti@histo.ufrj.br}
• 关键词：Vulvar lichen sclerosus ; Metalloproteinases (MMPs) ; Tissue inhibitors of metalloproteinases (TIMPs) ; Extracellular matrix
• 刊名：European Journal of Obstetrics and Gynecology and Reproductive Biology
• 出版年：2012
• 期刊代码：92_03012115
• 类别：med
• 出版时间：March, 2012
• 卷：161
• 期：1
• 页码：96-101
• 文件大小：785 K

摘要

Objectives

To evaluate the expression of different matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) in vulvar lichen sclerosus (LS), a chronic dermatosis in women, histologically characterized by a zone of collagen remodeling in the superior dermis.

Study design

Analysis of the expression of different MMPs (MMP-1, -2, -9 and -13) and TIMPs (TIMP-1 and -2) by reverse transcriptase-polymerase chain reaction (RT-PCR) in vulvar biopsies from patients with LS (n = 11), classified according to Hewitt histological criteria and compared with clinically normal vulvar tissue (n = 5), and the immunohistochemistry of MMP-2 and -9 and TIMP-1 and -2 distribution in the remodeling zone of LS (n = 31) and in clinically normal vulvar tissue (n = 28).

Results

Although no statistically significant difference between LS and normal skin groups at the mRNA level of MMP and TIMP transcripts was shown, an increase in the immunodistribution of MMP-2 and -9 and TIMP-1 and -2 in LS compared to normal vulvar skin was observed.

Conclusions

These results suggest that these molecules could be related to the process of cutaneous collagen remodeling in LS pathology.