DNA methylation imprints on the IG-DMR of the Dlk1–Gtl2 domain in mouse male germline
- 作者：Hitoshi Hiura ; Junichi Komiyama ; Motomu Shirai ; Yayoi Obata ; Hidehiko Ogawa ; Tomohiro Kono
- 关键词：Genomic imprinting ; Methylation ; DMR ; Germ cells ; Mouse
- 刊名：FEBS Letters
- 出版年：2007
- 期刊代码：70_00145793
- 类别：bio
- 出版时间：3 April 2007
- 卷：581
- 期：7
- 页码：1255-1260
- 文件大小：291 K
摘要
Mouse genomes show a large cluster of imprinted genes at the Dlk1–Gtl2 domain in the distal region of chromosome 12. An intergenic-differentially methylated region (IG-DMR) located between Dlk1 and Gtl2 is specifically methylated in the male germline; IG-DMR regulates the parental allele-specific expression of imprinted genes. Here, we show the resetting of IG-DMR methylation marks during male germ-cell differentiation. For parental allele-specific methylation analysis, polymorphisms were detected in a 2.6-kb IG-DMR in three mouse strains. Bisulfite methylation analysis showed erasure of the marks by E14 and re-establishment before birth. The IG-DMR methylation status was maintained in spermatogonia and spermatocytes of mature testes. The IG-DMR methylation status established before birth is thus maintained throughout the lifetime in the male germline.