Effects of hallucinogenic agents mescaline and phencyclidine on zebrafish behavior and physiology

详细信息	查看全文 | 推荐本文 |

• 作者：Evan J. Kyzar¹ ; Christopher Collins¹ ; Siddharth Gaikwad ; Jeremy Green ; Andrew Roth ; Louie Monnig ; Mohamed El-Ounsi ; Ari Davis ; Andrew Freeman ; Nicholas Capezio ; Adam Michael Stewart ; Allan V. Kalueff ; ^{avkalueff@gmail.com}
• 关键词：ANOVA ; analysis of variance ; DMSO ; dimethyl sulfoxide ; ELISA ; enzyme-linked immunosorbent assay ; Fps ; frames per second ; HPI ; Hypothalamic&ndash ; pituitary&ndash ; interrenal ; LSD ; lysergic acid diethylamide ; MDMA ; 3 ; 4-methylenedioxymethamphetamine ; NMDA ; N-
• 刊名：Progress in Neuro-Psychopharmacology and Biological Psychiatry
• 出版年：2012
• 期刊代码：222_02785846
• 类别：med
• 出版时间：27 April, 2012
• 卷：37
• 期：1
• 页码：194-202
• 文件大小：1015 K

摘要

Mescaline and phencyclidine (PCP) are potent hallucinogenic agents affecting human and animal behavior. As their psychotropic effects remain poorly understood, further research is necessary to characterize phenotypes they evoke in various animal models. Zebrafish (Danio rerio) are rapidly emerging as a new model organism for neuroscience research. Here, we examine the effects of mescaline (5-20 mg/l) and PCP (0.5-3 mg/l) in several zebrafish paradigms, including the novel tank, open field and shoaling tests. Mescaline and PCP dose-dependently increased top activity in the novel tank test, also reducing immobility and disrupting the patterning of zebrafish swimming, as assessed by ethograms. PCP, but not mescaline, evoked circling behavior in the open field test. At the highest doses tested, mescaline markedly increased, while PCP did not affect, zebrafish shoaling behavior. Finally, 20 mg/l mescaline did not alter, and 3 mg/l PCP elevated, whole-body cortisol levels. Overall, our studies indicate high sensitivity of zebrafish models to hallucinogenic compounds with complex behavioral and physiological effects.