Antibodies to tissue transglutaminase: comparison of ELISA and immunoprecipitation assay in the presence and in the absence of calcium ions

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• 作者：Nakachi ; Ken ; Swift ; Gillian ; Wilmot ; David ; Chapman ; Ceri ; Baker ; Stuart ; Powell ; Michael ; Furmaniak ; Jadwiga ; et. al.
• 关键词：Coeliac disease ; Tissue transglutaminase ; Autoantibodies ; Autoimmunity
• 刊名：Clinica Chimica Acta
• 出版年：2001
• 期刊代码：54_00098981
• 类别：med
• 出版时间：February, 2001
• 卷：304
• 期：1-2
• 页码：75-84
• 文件大小：161 K

摘要

Endomysial antibodies are characteristic of coeliac disease and tissue transglutaminase (tTG) has been identified as a major component of the endomysial antigen. tTG autoantibodies were measured in sera from patients with coeliac disease using ELISA based on guinea pig tTG and immunoprecipitation assay (IPA) based on ³⁵S-labelled human tTG produced in an in vitro transcription/translation system. In addition, the effect of calcium ions on the interaction between tTG autoantibodies in the two assays was studied. Under standard (i.e. Ca²⁺-free) conditions, 36/39 (92%) coeliac sera were positive for IgA tTG antibodies by ELISA and 34/39 (87%) sera were positive by IPA. Comparison of ELISA and IPA results showed three sera positive by ELISA but negative by IPA and one serum which was positive by IPA but negative by ELISA. Bland and Altman analysis of the correlation between the ELISA and IPA showed that the results for 37 out of 39 samples were in the agreement. The results by ELISA carried out without and with Ca²⁺were in good agreement (r