KOMA: ELISA-microarray calibration and data analysis based on kinetic signal amplification

详细信息	查看全文 | 推荐本文 |

• 作者：Pavlo Holenya¹ ; Florian Heigwer¹ ; Stefan Wö ; lfl ; ^{wolfl@uni-hd.de}
• 关键词：KOMA ; Kinetic Operating Microarray Analyzer ; ELISA ; enzyme-linked immunosorbent assay ; FDA ; Food and Drug Administration ; EDTA ; Ethylenediaminetetraacetic acid ; TMB ; 3 ; 3&prime ; 5 ; 5&prime ; -tetramethylbenzidine ; ATCC ; American Type Culture Collection ; DMEM
• 刊名：Journal of Immunological Methods
• 出版年：2012
• 期刊代码：57_00221759
• 类别：imm
• 出版时间：29 June, 2012
• 卷：380
• 期：1-2
• 页码：10-15
• 文件大小：701 K

摘要

Antibody microarrays with enzyme-linked immunosorbent technology are used for quantitative, simultaneous and high-throughput analysis of multiple proteins in a single probe. Kinetic detection can significantly improve precision and quantification range of microarray measurements. Here we present the open source software Kinetic Operating Microarray Analyzer (KOMA) that enables calibration and high-throughput analysis of quantitative microarray data collected using a time-resolved kinetic detection protocol of the enzymatic signal. This tool can also be helpful for analyzing data from any other analytical assays employing enzymatic signal amplification, in which a broader range of quantification is reached by the time-resolved recording of readouts. KOMA is open for download at together with a set of test raw data and requires R version 2.12 and Java RE version 6.0.