Regulation of lung surfactant secretion by microRNA-150

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• 作者：Tingting Weng ; Amarjit Mishra ; Yujie Guo ; Yang Wang ; Lijing Su ; Chaoqun Huang ; Chunling Zhao ; Xiao Xiao ; Lin Liu ; ^{lin.liu@okstate.edu}
• 关键词：P2X7R ; miR-150 ; Surfactant secretion
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2012
• 期刊代码：159_0006291x
• 类别：bio
• 出版时间：15 June, 2012
• 卷：422
• 期：4
• 页码：586-589
• 文件大小：356 K

摘要

P2X7 receptor (P2X7R) is a purinergic ion-channel receptor. We have previously shown that the activation of P2X7R in alveolar type I cells stimulates surfactant secretion in alveolar type II cells. In this study, we determined whether miR-150 regulates P2X7R-mediated surfactant secretion. The miR-150 expression level in alveolar type II cells was much higher than alveolar type I cells, which was inversely correlated with the P2X7R protein level. An adenovirus expressing miR-150 significantly reduced the P2X7R protein expression in E10 cells, an alveolar type I cell line. Furthermore, pre-treatment of E10 cells with the adenovirus reduced the surfactant secretion induced by E10 cell conditioned medium. Our study demonstrates that miR-150 regulates surfactant secretion through P2X7R.