The proteome of the human neuroblastoma cell line SH-SY5Y: An enlarged proteome
- 作者：Kambiz Gilany ; Roos Van Elzen ; Kim Mous ; Edmond Coen ; Walter Van Dongen ; Stefaan V ; amme ; Kris Gevaert ; Evy Timmerman ; Joë ; l V ; ekerckhove ; Sylvia Dewilde ; Xaveer Van Ostade ; Luc Moens
- 关键词：Proteome analysis ; N-terminal COFRADIC ; 2D-LC-MALDI-TOF/TOF-MS ; SDS-PAGE nano-LC– ; MS/MS ; 2D-PAGE
- 刊名：Biochimica et Biophysica Acta - Proteins and Proteomics
- 出版年：2008
- 期刊代码：161_15709639
- 类别：bio
- 出版时间：July-August 2008
- 卷：1784
- 期：7-8
- 页码：983-985
- 文件大小：188 K
摘要
The human neuroblastoma cell line SH-SY5Y (ATCC: CRL-2266) is widely used as a neural cellular model system. The hitherto existing proteome data (115 proteins) are here extended. A total of 1103 unique proteins of this cell line were identified using 2D-LC combined with MALDI-TOF/TOF-MS, SDS-PAGE with nano-LC–MS/MS, N-terminal COFRADIC analysis with nano-LC–MS/MS and 2D-PAGE with MALDI-TOF/TOF-MS peptide mass fingerprinting. The obtained proteome profile of this cell line is discussed.