Highly sensitive detection of alkaline phosphatase using molecular beacon probes based on enzymatic polymerization

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• 作者：Changbei Ma^a ; ^b ; ^{lhlmcb@yahoo.com.cn}
• 关键词：Alkaline phosphatase ; Inhibitor ; Molecular beacon
• 刊名：Molecular and Cellular Probes
• 出版年：2012
• 期刊代码：104_08908508
• 类别：imm
• 出版时间：June, 2012
• 卷：26
• 期：3
• 页码：113-115
• 文件大小：276 K

摘要

We have developed a new methodology for highly sensitive alkaline phosphatase assay using molecular beacon probes. No incubation step is needed to obtain a limit of detection for ALP of 2聽脳聽10^鈭?6聽M. Furthermore, ALP inhibition by the inhibitor okadaic acid is shown, demonstrating the potential for high-throughput screening for inhibitors.