Determination of patulin in fruit juices using HPLC-DAD and GC-MSD techniques

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• 作者：Athanasios Moukas ; Vasiliki Panagiotopoulou ; Panagiota Markaki
• 关键词：HPLC-DAD ; GC-MSD ; Patulin ; Fruit juices ; Risk assessment
• 刊名：Food Chemistry
• 出版年：2008
• 期刊代码：48_03088146
• 类别：abs
• 出版时间：15 August 2008
• 卷：109
• 期：4
• 页码：860-867
• 文件大小：151 K

摘要

A high performance liquid chromatography with a diode-array detector (HPLC-DAD) and a gas chromatography with a mass spectrometer (GC-MSD) are described for the determination of patulin (PAT) in apple juice. The limits of detection (DL) and quantification (QL) for the HPLC-DAD and GC-MSD method were found to be (DL = 0.23 μg kg⁻¹ QL = 1.2 μg kg⁻¹) and (DL = 5.8 μg kg⁻¹ and QL = 13.8 μg kg⁻¹), respectively. The recovery factors for HPLC-DAD and GC-MSD were found to be 99.5%(RSD%= 0.73) and 41%(RSD%= 10.03), respectively. The HPLC-DAD method was used to determine the occurrence of PAT in 90 samples of fruit juices.