Methanol extract of Desmodium gangeticum DC root mimetic post-conditioning effect in isolated perfused rat heart by stimulating muscarinic receptors

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• 作者：Gino A Kurian^a ; ^{ginokurian@hotmail.com} ; Jose Paddikkala^b
• 关键词：Myocardial ischemia reperfusion ; Ischemic precondition ; Ischemic post conditioning ; Acetyl choline ; Desmodium gangeticum ; Muscarinic receptor
• 刊名：Asian Pacific Journal of Tropical Medicine
• 出版年：2012
• 期刊代码：pca68_19957645
• 类别：med
• 出版时间：June, 2012
• 卷：5
• 期：6
• 页码：448-454
• 文件大小：434 K

摘要

Objective

To evaluate pharmacological mimetic action of herbal extract Desmodium gangeticum (DG) roots on ischemia reperfusion injury.

Methods

With the help of Langendroff perfusion technique, ischemic post condition (POC) mimetic action of DG methanol root extract was evaluated and compared by using standard drugs that acts as muscarinic receptor agonist and antagonist, namely acetylcholine (Ach) and atropine (Atr) respectively in an isolated rat heart.

Results

The physiological parameters like left ventricular developed pressure, end diastolic pressure and working index of isolated rat heart showed significant recovery in DG root extract administrated rat heart, similar to the recovery by POC. Kymogram results showed muscarinic receptor agonist like action for DG methanol root extract, confirmed in rat heart by muscarnic receptor agonist (acetylcholine) and anatoginst (atropine). Administration of DG root extract prior to reperfusion showed better antioxidant status in myocardial tissue homogenate and mitochondrial, complemented by the levels of cardiac specific marker proteins in myocardial tissue and perfusate. Even though DG methanol root extract mimics its action similar to that of Ach, the myocardial protection mediated by the extract was superior to Ach, due to the presence of antioxidants in the crude extract.

Conclusions

DG methanol root extract provides myocardial protection towards IRI by stimulating muscarinic receptors.