Evaluation of plasmatic MMP-8, MMP-9, TIMP-1 and MPO levels in obese and lean women

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• 作者：Vanessa L. Andrade^a ; Eveline Petruceli^a ; Vanessa A. Belo^b ; Claudia M. Andrade-Fernandes^c ; Cristiane V. Caetano Russi^c ; Adriana Ap. Bosco^a ; José ; Eduardo Tanus-Santos^b ; Valeria Cristina Sandrim^a ; ^{valeria.sandrim@santacasabh.org.br} ; ^{valsandrim@yahoo.com.br}
• 关键词：MMP-8 ; Neutrophil collagenase ; MMP-9 ; TIMP-1 ; Obesity ; MPO ; Neutrophil activation
• 刊名：Clinical Biochemistry
• 出版年：2012
• 期刊代码：55_00099120
• 类别：med
• 出版时间：April, 2012
• 卷：45
• 期：6
• 页码：412-415
• 文件大小：270 K

摘要

Objectives

To compare the plasma concentrations of matrix metalloproteinase (MMP)-9, tissue inhibitor of MMP (TIMP)-1, MMP-8, and myeloperoxidase (MPO) for obese and lean women.

Design and methods

We recruited 30 lean and 36 obese women without comorbidities. The MMP-9, TIMP-1, and MMP-8 levels were measured using enzyme-linked immunosorbent assay (ELISA). MPO activity was assessed by a colorimetric assay.

Results

Obese women had higher MMP-9 levels and MMP-9:TIMP-1 ratios than lean women. Conversely, the MMP-8 levels and MMP-8:TIMP-1 ratios in the obese women were significantly lower than those in the lean women despite neutrophil activation, which was assessed by MPO activity.

Conclusion

We observed that MMP-9 and MMP-8 had distinct profiles, which suggested that these 2 enzymes play different roles in obesity.